Sweden’s New Covid-19 Testing Method Promises To Be Cheaper, Faster And Accurate

According to the researchers, the most important keys to the method's success are virus inactivation procedure and a new formulation of the solution used to collect and transport the sample material.

A method for fast, cheap, yet accurate testing for Covid-19 infection has been developed by a team of researchers. The method simplifies and frees the testing from expensive reaction steps, enabling upscaling of the diagnostics.

This makes the method particularly attractive for places and situations with limited resources. It is equally interesting for repeated testing and for moving resources from expensive diagnostics to other parts of the care chain.

The study, led by researchers at the Karolinska Institutet, was published in the journal Nature Communications.

“We started working on the issue of developing a readily available testing method as soon as we saw the developments in Asia and southern Europe, and before the situation reached crisis point in Sweden,” says principal investigator Bjorn Reinius, research leader at the Department of Medical Biochemistry and Biophysics at Karolinska Institutet.

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“Our method was effectively finished already by the end of April, and we then made all the data freely available online.”

The spread of the new coronavirus at the end of 2019 in China’s Wuhan region quickly escalated into a global pandemic. The relatively high transmission rate and a large number of asymptomatic infections led to a huge, worldwide need for fast, affordable, and effective diagnostic tests that could be performed in clinical as well as non-clinical settings.

Established diagnostic tests for Covid-19 are based on the detection of viral RNA in patient samples, such as nasal and throat swabs, from which RNA molecules must then be extracted and purified.

RNA purification constitutes a major bottleneck for the testing process, requiring a great deal of equipment and logistics as well as expensive chemical compounds.

Making the current methods simpler without markedly compromising their accuracy means that more and faster testing can be carried out, which would help to reduce the rate of transmission and facilitate earlier-stage care.

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Methods developed by the the cross-departmental research group at Karolinska Institutet, completely circumvent the RNA-extraction procedure. Once the patient sample inactivated by means of heating, it renders the virus particles ineffective.

According to the researchers, the most important keys to the method’s success are both the above virus inactivation procedure and a new formulation of the solution used to collect and transport the sample material taken from the patients.

“By replacing the collection buffer with simple and inexpensive buffer formulations, we can enable viral detection with high sensitivity directly from the original clinical sample, without any intermediate steps,” says Dr. Reinius.

Institutions and research groups around the world have shown great interest in the method since the first version of the scientific article was published on the preprint server medRxiv. The article was read more than 15,000 times even before it was peer-reviewed by other researchers in the field and officially published in Nature Communications.

“Thanks to the low cost and the simplicity of the method, it becomes a particularly attractive option at sites and in situations with limited resources but a pressing need to test for Covid-19,” says Dr. Reinius.

“I would certainly like to see that this test used in Sweden too, for example for cheap periodic testing of asymptomatic people to eliminate the spread of infection”, he adds.

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